Part:BBa_K2913009
PfnrF8
This is a hypoxia-inducible promoter to restrict gene expression specifically in the hypoxic environment of tumors. The transcriptional activation promoted by the PfnrF8 needs a specific transcription factor called FNR, which can bind to oxygen. When the oxygen level is at a normal range in the internal environment, the transcription factor FNR will associate with oxygen, leading to its impeded binding to the PfnrF8 promoter, and reduce the transcription of its downstream gene. However, in the hypoxic environment of tumors, FNR will disassociate with oxygen and activate its downstream gene.
Usage and Biology
result
Written by iGEM19_NEFU_China
We use this part to feel the hypoxia environment in our case.
The series of concentrations of sodium sulfite (0, 0.25, 0.5, 0.75 and 1 g/l Na2SO3) can make hypoxia environment (Fig.a). Under the PfnrF8 promoter, the activity of lacZ can be seen to rise markedly from 0 g/l to 1 g/l Na2SO3 (Fig.b). This promoter can respond our hypoxia environment regularly.See more information on iGEM19_NEFU_China's Result PAGE
References
[1] Ryan, R.M., et al., Bacterial delivery of a novel cytolysin to hypoxic areas of solid tumors. Gene Ther, 2009. 16(3): p. 329-39.
[2] Bell, A. and S. Busby, Location and orientation of an activating region in the Escherichia coli transcription factor, FNR. Mol Microbiol, 1994. 11(2): p. 383-90.
[3] Moser, F., et al., Dynamic control of endogenous metabolism with combinatorial logic circuits. Mol Syst Biol, 2018. 14(11): p. e8605.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 21
Illegal NheI site found at 44 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
None |